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Molecular-biological investigation

PCR enables a rapid and exact detection of the pathogenic agents in the biological material using their specific genetic features.

The method is based on the determination of a specific DNA sequence of the pathogenic agent using complementary primers of DNA matrix, its amplification and identification by electrophoresis.

Polymerase chain reaction was discovered more than 30 years ago by A. Cornberg, which together with another Nobel laureate - Dj. Lederberg expressed their idea that, with this technique can be obtained large amounts of DNA. This idea was implemented more than 20 years later by Carry Mullis, Nobel Prize in Chemistry, 1993. Thus, C. Mullis, based on the natural process of DNA replication, developed the method that permits to obtain copies of certain portions of genes in unlimited quantities in a test-tube (in vitro). This method was called polymerase chain reaction. It has become one of the most convenient and useful methods of DNA diagnostics.

The first publication about the practical application of PCR appeared in 1985. Since then, PCR technology has undergone multiple changes. The scientists have developed procedures that allow to improve significantly the opportunities of initially proposed PCR method and reduce its deficiencies.

Molecular-biological methods, especially PCR, have made a breakthrough in diagnosing of urogenital inflammation. PCR has priority over other methods by high sensitivity of about 80–100%.


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